A rapid method for immunofluorescent staining of paraffin sections using iron-containing protein A microspheres.

Immunofluorescence staining of paraffin sections: creating DAB staining like virtual digital images using CMYK color conversion.

Crystal violet treatment of formalin fixed paraffin embedded tissue slides greatly reduces the endogenous autofluorescence, and allows immunofluorescence (IF) staining with FITC or Alexa488 conjugated antibodies. Using cold CCD camera to capture the fluorescence images makes this staining method very sensitive. Here we show that combination of IF with the simultaneous recording of crystal viole...

Immunofluorescent Staining: the Fluorescent Antibody Method.

I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining................

Comparison of Three Staining Methods of Vibratome and Paraffin Sections in Preserving Thickness of Rat Hippocampal Sections

Purpose: To determine the optimal technique for maintaining hippocampus vibratome sections volume. Materials and Methods: This experimental study was performed on three rat hippocampus. The first hippocampus was embedded in paraffin and using rotary microtome forty sections were cut in a nominal thickness of 100 micrometer. They were then ordinary stained. The two others were cut into 80 sectio...

A Rapid Method of Paraffin Embedding

A Routine Method for Obtaining High Contrast without Staining Sections

Immersion of tissue in aqueous uranyl acetate be-for giving some degree of contrast to components fore embedding is commonly practiced to increase such as membranes when they extend through the the contrast on sections which are subsequently thickness of the section parallel to the electron stained (2, 3, 5). The treatment is only satisfactory beam. A simple modification of current practice 540